Salmonella Typhi and Plasmodium falciparum Co-infection in a 12-year Old Girl with Haemoglobin E Trait from a Non-malarious Area in Bangladesh

A 12-year old girl from Uttar Badda, Dhaka, Bangladesh, was admitted to the Dhaka Hospital of ICDDR,B, with a 23-day history of fever and diarrhoea. After admission, she was treated for culture-proven Salmonella Typhi-associated infection and was discovered to be heterozygous for haemoglobin E. Despite treatment with appropriate antibiotics, the patient's condition did not improve, prompting further investigation, which revealed malaria due to Plasmodium falciparum. Dhaka is considered a malaria-free zone, and the patient denied recent travel outside Dhaka. Subsequently, the patient recovered fully on antimalarial therapy

Screening for cervical cancer (By VIA Test) among selected garments worker in Chattogram, Bangladesh

Background: Bangladesh is a densely populated country of South East Asia with low resource setting where cervical cancer is the 2nd leading cause of female cancer. In more than 80% cases are diagnosed at advanced and inoperable stage. Regarding socio demographic context of this country VIA has been introduced as a screening method for cervical cancer which is most simple, cost effective, and acceptable test for all women. In Bangladesh among 3 million garment workers more than 80% are women. The objective of this study was to identify prevalence of VIA positive cases among garment workers. So that it can reduce the incidence of cervical cancer in Bangladesh. Methods: It was a cross–sectional observational study conducted in some selected garment factories in Chattogram city of Bangladesh from January 2021 to July 2021, where we enrolled 534 female workers for VIA test. Results: Among all the respondents 56% were 30 years or younger, 38% were aged between 31 to 40 years. Among 534 participants, 44.9% completed primary education, 37.3% were smoker and 34.5% had their children at early age. Majority (86.7%) had excessive whitish discharge. Post coital bleeding and irregular bleeding was 2.6% and 2.2% respectively. Considering awareness, 61.8% had idea about cervical cancer, only 1.1% had undergone VIA test in the past. In our study we found 2.4% of participants were VIA positive cases. Conclusions: It is important to include the garment workers, while making public health policies and implementation of cervical cancer control program

Salmonella typhi and Plasmodium falciparum co-infection in a 12-year old girl with haemoglobin E trait from a non-malarious area in Bangladesh

A 12-year old girl from Uttar Badda, Dhaka, Bangladesh, was admitted to the Dhaka Hospital of ICDDR,B, with a 23-day history of fever and diarrhoea. After admission, she was treated for culture-proven Salmonella typhi -associated infection and was discovered to be heterozygous for haemoglobin E. Despite treat-ment with appropriate antibiotics, the patient\u2032s condition did not improve, prompting further investiga-tion, which revealed malaria due to Plasmodium falciparum . Dhaka is considered a malaria-free zone, and the patient denied recent travel outside Dhaka. Subsequently, the patient recovered fully on antimalarial therapy

Cortical excitability correlates with seizure control and epilepsy duration in chronic epilepsy

OBJECTIVE: Cortical excitability differs between treatment responders and nonresponders in new‐onset epilepsy. Moreover, during the first 3 years of epilepsy, cortical excitability becomes more abnormal in nonresponders but normalizes in responders. Here, we study chronic active epilepsy, to examine whether cortical excitability continues to evolve over time, in association with epilepsy duration and treatment response. METHODS: We studied 28 normal subjects, 28 patients with moderately controlled epilepsy (≤4 seizures per year) and 40 patients with poorly controlled epilepsy (≥20 or more seizures per year). Resting motor threshold (RMT), active motor threshold (AMT), short‐interval intracortical inhibition (SICI), intracortical facilitation (ICF) and cortical silent period (CSP) were measured, using transcranial magnetic stimulation (TMS). Disease and treatment covariates were collected (age at onset of epilepsy, epilepsy duration, number of drugs prescribed, total drug load, sodium channel drug load). RESULTS: RMT and AMT were higher in patients than in normal subjects; RMT and AMT were higher in poorly controlled than moderately controlled patients. ICF at 12 msec and 15 msec were lower in poorly controlled patients than in normal subjects. Long‐interval intracortical inhibition (LICI) at 50 msec was higher in poorly controlled compared to moderately controlled patients. These differences were not explained by antiepileptic drug (AED) treatment or duration of epilepsy. RMT and AMT increased with duration in the poorly controlled group, but did not increase with duration in the moderately controlled group. INTERPRETATION: Cortical excitability differs markedly between moderately controlled and poorly controlled patients with chronic epilepsy, not explained by disease or treatment variables. Moreover, the evolution of cortical excitability over time differs, becoming more abnormal in the poorly controlled group

Multiple mechanisms shape the relationship between pathway and duration of focal seizures

A seizure’s electrographic dynamics are characterized by its spatiotemporal evolution, also termed dynamical ‘pathway’, and the time it takes to complete that pathway, which results in the seizure’s duration. Both seizure pathways and durations have been shown to vary within the same patient. However, it is unclear whether seizures following the same pathway will have the same duration or if these features can vary independently. We compared within-subject variability in these seizure features using (i) epilepsy monitoring unit intracranial EEG (iEEG) recordings of 31 patients (mean: 6.7 days, 16.5 seizures/subject), (ii) NeuroVista chronic iEEG recordings of 10 patients (mean: 521.2 days, 252.6 seizures/subject) and (iii) chronic iEEG recordings of three dogs with focal-onset seizures (mean: 324.4 days, 62.3 seizures/subject). While the strength of the relationship between seizure pathways and durations was highly subject-specific, in most subjects, changes in seizure pathways were only weakly to moderately associated with differences in seizure durations. The relationship between seizure pathways and durations was strengthened by seizures that were ‘truncated’ versions, both in pathway and duration, of other seizures. However, the relationship was weakened by seizures that had a common pathway, but different durations (‘elasticity’), or had similar durations, but followed different pathways (‘semblance’). Even in subjects with distinct populations of short and long seizures, seizure durations were not a reliable indicator of different seizure pathways. These findings suggest that seizure pathways and durations are modulated by multiple different mechanisms. Uncovering such mechanisms may reveal novel therapeutic targets for reducing seizure duration and severity

Intracranial EEG structure-function coupling predicts surgical outcomes in focal epilepsy

Alterations to structural and functional brain networks have been reported across many neurological conditions. However, the relationship between structure and function -- their coupling -- is relatively unexplored, particularly in the context of an intervention. Epilepsy surgery alters the brain structure and networks to control the functional abnormality of seizures. Given that surgery is a structural modification aiming to alter the function, we hypothesized that stronger structure-function coupling preoperatively is associated with a greater chance of post-operative seizure control. We constructed structural and functional brain networks in 39 subjects with medication-resistant focal epilepsy using data from intracranial EEG (pre-surgery), structural MRI (pre-and post-surgery), and diffusion MRI (pre-surgery). We investigated pre-operative structure-function coupling at two spatial scales a) at the global iEEG network level and b) at the resolution of individual iEEG electrode contacts using virtual surgeries. At global network level, seizure-free individuals had stronger structure-function coupling pre-operatively than those that were not seizure-free regardless of the choice of interictal segment or frequency band. At the resolution of individual iEEG contacts, the virtual surgery approach provided complementary information to localize epileptogenic tissues. In predicting seizure outcomes, structure-function coupling measures were more important than clinical attributes, and together they predicted seizure outcomes with an accuracy of 85% and sensitivity of 87%. The underlying assumption that the structural changes induced by surgery translate to the functional level to control seizures is valid when the structure-functional coupling is strong. Mapping the regions that contribute to structure-functional coupling using virtual surgeries may help aid surgical planning

Diminished circadian and ultradian rhythms in pathological brain tissue in human in vivo

Chronobiological rhythms, such as the circadian rhythm, have long been linked to neurological disorders, but it is currently unknown how pathological processes affect the expression of biological rhythms in the brain. Here, we use the unique opportunity of long-term, continuous intracranially recorded EEG from 38 patients (totalling 6338 hours) to delineate circadian and ultradian rhythms in different brain regions. We show that functional circadian and ultradian rhythms are diminished in pathological tissue, independent of regional variations. We further demonstrate that these diminished rhythms are persistent in time, regardless of load or occurrence of pathological events. These findings provide the first evidence that brain pathology is functionally associated with persistently diminished chronobiological rhythms in vivo in humans, independent of regional variations or pathological events. Future work interacting with, and restoring, these modulatory chronobiological rhythms may allow for novel therapies